Agroinoculation of Citrus tristeza virus Causes Systemic Infection and Symptoms in the Presumed Nonhost Nicotiana benthamiana
نویسندگان
چکیده
منابع مشابه
A genetic system for Citrus Tristeza Virus using the non-natural host Nicotiana benthamiana: an update
In nature Citrus tristeza virus (CTV), genus Closterovirus, infects only the phloem cells of species of Citrus and related genera. Finding that the CTV T36 strain replicated in Nicotiana benthamiana (NB) protoplasts and produced normal virions allowed development of the first genetic system based on protoplast transfection with RNA transcribed from a full-genome cDNA clone, a laborious and unce...
متن کاملCitrus tristeza virus-host interactions
Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...
متن کاملThe Complex Genetics of Citrus tristeza virus
The 2000 x 11 nm long bipolar flexuous filamentous particles of Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) (Figure 1) contain a single-stranded positive-sense RNA genome of 19.3 kb, which is encapsidated in two different capsid proteins that coat the opposite ends of the virions [1, 2]. CTV is the largest identified RNA virus infecting plants and the second larges...
متن کاملSugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana
Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1)...
متن کاملVirus Infection Suppresses Nicotiana benthamiana Adaptive Phenotypic Plasticity
Competition and parasitism are two important selective forces that shape life-histories, migration rates and population dynamics. Recently, it has been shown in various pathosystems that parasites can modify intraspecific competition, thus generating an indirect cost of parasitism. Here, we investigated if this phenomenon was present in a plant-potyvirus system using two viruses of different vi...
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ژورنال
عنوان ژورنال: Molecular Plant-Microbe Interactions®
سال: 2011
ISSN: 0894-0282,1943-7706
DOI: 10.1094/mpmi-05-11-0110